| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1213-1 | ||||
Resumo:Agaricomycetes has been studied in the search for several secondary metabolites, and its anti-inflammatory, antifungal, antioxidant, antiviral, antimicrobial and antitumor potential is already known. In addition to bioactive compounds, they also have the ability to secrete hydrolytic enzymes of industrial interest, such as cellulase, amylase, protease and lipase. Despite the great Amazonian diversity, there are still few studies related to macroscopic fungi, specifically Agaricomycetes from this biome. Thus, the objective of this work was to evaluate the antimicrobial and enzymatic activity of Agaricomycetes fungi from the Humaitá Forest Reserve, Acre, Brazil. Basidiomes were collected and the macroscopic and microscopic characteristics were analyzed for identification. To evaluate the antimicrobial activity, extracts of the basidiome and mycelium were produced and evaluated against pathogenic bacteria and fungi using the microdilution test. The production of cellulase, protease, amylase and lipase enzymes was evaluated using the cup plate test by isolated basidiomycetes. The 55 Agaricomycetes collected were classified in the orders Agaricales (45.45%), Polyporales (41.81%), Auriculariales (9.09%) and Hymenochaetales (3.63%), distributed in 16 families, 23 genera and 16 species. The species Clitopilus prunulus is being reported as the first occurrence for the State of Acre, Brazil. The 10 Agaricomycetes collected were used to produce basidiome extracts and 18 to produce mycelium extract. The minimum inhibitory concentration for basidiome extracts ranged from 0.62 to 10 mg/mL and for mycelia extracts ranged from 0.62 to 20 mg/mL. The specimens that showed the lowest microbicidal values in the extracts were Flabellophora sp. 5.383, Agaricus sp. 5.389, Polyporaceae 5.372, Polyporales 5.417, and for mycelium extract specimens Agaricales 5.367(3), Marasmius sp. 5392, with 2.5 mg/mL. Of the 24 Agaricomycetes isolated, 15 showed enzymatic production. The most frequent enzymatic activity of the fungi analyzed was cellulolytic (50%), with the best results for the isolates Polyporaceae 5.418 and Agaricales 5.381, followed by the enzyme protease (29.16%), with the largest halos for Pluteus sp. 5.365 and Agaricales 5.367(2) and 5.388. This study contains the first report of enzymatic activity for the genus Polyporus, and for lipase production, for the genera Polyporus, Marasmius and Ceriporia. In view of the limited amount of information in the literature on enzymatic and antimicrobial activity, further studies on Agaricomycetes from the Amazon are needed. Palavras-chave: Agaricales, Cellulase, Ceriporia, Polyporales, Polyporus Agência de fomento:Capes | |||||